A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

This book focuses on the developmental analysis of brain-culture-environment dynamics and argues that this dynamic is interactive and reciprocal; brain and culture co-determine each other. As a whole, this book refutes any unidirectional conception of the brain-culture dynamic, as each is influenced by and modifies the other. To capture the ubiquitous reach and significance of the mutually dependent and co-productive brain-culture system, the metaphor of biocultural co-constructivism is invoked. Distinguished researchers from cognitive neuroscience, cognitive psychology, and developmental psychology review the evidence in their respective fields. A special focus of the book is its coverage of the entire human lifespan.