In Aliens vs. Predator players will have the chance to take the role of the three infamous species; the Colonial Marine, the Predator and the Alien. Each of the three species has its very own distinct story-driven single-player campaign mode that interweaves with the campaigns of the other two species.

A rerelease of Aliens versus Predator was released in 2010. Retitled as Aliens versus Predator Classic 2000, this version of the game has been updated to work on modern computers, as well as support for the Xbox 360 controllers. Moreoever, this release includes all the levels from the original AvP Gold Edition, and the extra content of the Millennium Addon Pack. Supporting only singleplayer initially, multiplayer functionality has since been added. This release can be purchased through Valves Steam digital distribution service. A patch released on 19 January, 2010 via Steam added proper widescreen support, optional unlimited saves, a locked frame rate, mouse improvements, and other updates to the game.

Chosen for their ability to kill without conscience, a group of killers, some trained and some who are not, must endeavour the alien race of predators that have set out to target them as prey. Dropped into the vast jungle of a distant world, these human predators must learn just who, or what, they are up against, and that their ability, knowledge and wits are tested to the limits in the battle of survival of kill or be killed.

High above the arctic circle, on a remote Canadian island, a hunter shoots what he believes is a polar bear. He is mistaken. His Inuit guide has never seen an animal like this before. The killing unleashes an investigation by the Canadian authorities. But no-one can tell exactly what he has shot.

During an archaeological expedition on Bouvetшya Island in the Antarctic ocean, a team of archaeologists and other scientists find themselves caught up in a battle between the two legends. Soon, the team realise that only one species can win.

In Gunnison County, a spacecraft crashes in the woods bringing a powerful hybrid Alien hosted inside the pilot Predator. The local Buddy Benson and his son Sam are hunting in the forest and witness the crash, but they are chased and killed by the Alien. Meanwhile another Predator lands on the spot seeking out the Alien and destroying the evidences of their presence on Earth. The dwellers of the town find themselves in the middle of a battlefield between the two deadly extraterrestrial creatures, and the small group of survivors splits the leadership between Sheriff Eddie Morales and the bad-boy Dallas Howard that have different opinions about the best means to escape from the beings.